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2017

Степанов В.А., Харьков В.Н., Вагайцева К.В., Бочарова А.В., Казанцев А.Ю., Попович А.А., Хитринская И.Ю.
Генетика. 2017. Т. 53. № 11. С. 1254-1266
DOI: 10.7868/S0016675817110121

Исследовано генетическое разнообразие популяций коренного населения Северной Евразии по панели генетических маркеров кандидатных генов адаптации к холодному климату. Обнаружен высокий уровень внутрипопуляционного и межпопуляционного разнообразия в исследованных популяциях. Сравнительный анализ полученных данных с данными по мировым популяциям из проектов “1000 геномов” и HGDP выявил корреляции генетического разнообразия кандидатных генов адаптации к холодному климату с ключевыми климатическими характеристиками, а также обнаружил рост генетического разнообразия маркеров этой группы генов по мере удаления от экватора, т.е. в ходе расселения человека из Африки. Методом поиска крайних эмпирических значений коэффициента генетического разнообразия выявлены сигналы направленного отбора для маркеров шести генов адаптации к холоду - MYOF, LONP2, IFNL4, MKL1, SLC2A12 и CPT1A. Данные обсуждаются в рамках гипотезы деканализации геном-феномных отношений под действием естественного отбора в ходе расселения человека по территории земного шара.

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Tarasenko N.V., Goncharova I.A., Markov A.V., Kondrat’eva E.I.
Russian Journal of Genetics. 2017. 53(8), 923–929.
DOI: 10.1134/S1022795417070110

The genetic structure of susceptibility to type 1 diabetes (T1D) in the population of Tomsk was studied. We had a group of T1D patients (N = 285) and a population sample (N = 300) and we studied 58 SNPs localized in the 47 genes which products are involved in various metabolic pathways and processes as fibrogenesis, endothelial dysfunction, and inflammation. Genotyping was performed by mass spectrometry using the Sequenom MassARRAY system (United States). We compared the group of T1D patients and the population sample and found an association with the predisposition to disease for seven markers: rs3765124 of the ADAMDEC1 gene, genotype AA (p = 0.004), allele A (p = 0.033); rs1007856 of the ITGB5 gene, genotype TT (p = 0.015), allele T (p = 0.036); rs20579 of the LIG1 gene, genotype CC (p = 0.004), allele C (p = 0.002); rs12980602 of the IFNL2 gene, allele C (p = 0.029); rs4986819 of the PARP4 gene, allele C (p = 0.044); rs1143674 of the ITGA4 gene genotype GG (p = 0.002); rs679620 of the MMP3 gene, genotype AA (p = 0.008). Thus, the products of genes associated with T1D belong to different molecular classes: metalloproteases (ADAMDEC1, MMP3), cytokines (IL28A), cell surface receptors (ITGA4), adhesion molecules (ITGB5), DNA ligases (LIG1), and ribosyltransferase enzymes (PARP4). The ADAMDEC1, ITGA4, and ITGB5 genes belong to two biological processes: cell communication and signal transduction. The LIG1 and PARP4 genes regulate the metabolism of nucleic acids, MMP3 is involved in the regulation of protein metabolism, and the IFNL2 is involved in the immune response.

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Тарасенко Н.В., Гончарова И.А., Марков А.В., Кондратьева Е.И.
Генетика. 2017. Т. 53. № 8. С. 973-980.
DOI: 10.7868/S0016675817070116

В настоящей работе была исследована генетическая структура подверженности сахарного диабета 1-го типа (СД1) в популяции г. Томска. В группе больных СД1 (N = 285) и популяционной выборке (N = 300) изучено 58 однонуклеотидных полиморфизмов, локализованных в 47 генах, продукты которых участвуют в различных метаболических путях и вовлечены в процессы фиброгенеза, эндотелиальную дисфункцию, иммунный ответ и воспаление. Генотипирование выполнено методом масс-спектрометрии на приборе ""Sequenom MassARRAY"" (США). В результате выявлена ассоциация с СД1 для семи генетических маркеров: rs3765124 гена ADAMDEC1 генотип AA (р = 0.004) и аллель A (р = 0.033); rs1007856 гена ITGB5 генотип TT (р = 0.015) и аллель T (р = 0.036); rs20579 гена LIG1 генотип CC (р = 0.004) и аллель C (р = 0.002); rs12980602 гена IFNL2 аллель C (р = 0.029); rs4986819 гена PARP4 аллель C (р = 0.044); rs1143674 гена ITGA4 генотип GG (р = 0.002); rs679620 гена MMP3 генотип AA (р = 0.008). Продукты генов, для которых получена ассоциация с СД1, принадлежат к различным молекулярным классам: металлопротеазы (ADAMDEC1, MMP3), цитокины (IL28A), рецепторы клеточной мембраны (ITGA4), молекулы адгезии (ITGB5), ДНК-лигазы (LIG1), ферменты рибозилтрансферазы (PARP4). Гены ADAMDEC1, ITGA4, ITGB5 участвуют в двух биологических процессах: передаче сигнала и межклеточных взаимодействиях. Гены LIG1, PARP4 регулируют метаболизм нуклеиновых кислот, MMP3 вовлечен в регуляцию метаболизма белков, IFNL2 в иммунный ответ.

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Sazhenova E.A., Nikitina T.V., Skryabin N.A., Minaycheva L.I., Ivanova T.V., Nemtseva T.N. , Yuriev S.Yu., Evtushenko I.D., Lebedev I.N.
Russian Journal of Genetics. 2017. 53(3), 376-387.
DOI: 10.1134/S1022795417020090

An analysis of differential methylation of 47 imprinted genes in placenta tissues of spontaneous abortions at the first trimester of pregnancy from women with recurrent pregnancy loss or with one sporadic abortion was performed using the DNA-microarray approach. We showed that epimutations of the imprinted genes were registered significantly more often in abortions from women with recurrent miscarriage in contrast to the embryos from women with sporadic pregnancy loss with frequency of 6.2 and 3.7% per locus, respectively (p < 0.01). The predominant type of epimutation appeared to be a postzygotic hypomethylation of the imprinted genes on chromosomes of maternal origin, which was observed in the examined samples in 5.1 and 2.89% of cases, respectively. Replicative study of the methylation status of seven imprinted genes (DLK1, PEG10, PLAGL1, KCNQ1OT1, PEG3, GRB10, and PEG1/MEST) in the enlarged embryo samples supported the results of microarray analysis in respect to both epimutation frequency and predominance of somatic hypomethylation of maternal alleles. It was also demonstrated that pregnancy loss was associated with multilocus methylation defects of imprinted genes, the frequency of which was also significantly increased in the placental tissues of spontaneous abortions in women with recurrent miscarriage.

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Саженова Е.А., Никитина Т.В., Скрябин Н.А., Минайчева Л.И., Иванова Т.В., Немцева Т.Н., Юрьев С.Ю., Евтушенко И.Д., Лебедев И.Н.
Генетика. 2017. Т. 53. № 3. С. 364-377
DOI: 10.7868/S0016675817020096

В плацентарных тканях спонтанных абортусов I триместра беременности от женщин с привычным невынашиванием беременности или с одним спорадическим абортом с использованием ДНК-микрочипов проведен анализ дифференциального метилирования 47 импринтированных генов. Показано, что у абортусов от женщин с привычным невынашиванием беременности в отличие от эмбрионов от женщин со спорадической потерей беременности статистически значимо чаще регистрируются эпимутации импринтированных генов с частотой 6.2 и 3.7% на локус соответственно (р < 0.01). Преобладающим типом эпимутаций оказалось постзиготическое гипометилирование импринтированных генов на хромосомах материнского происхождения, составившее в исследованных группах 5.1 и 2.9% на локус соответственно. Репликативное исследование статуса метилирования семи импринтированных генов (DLK1, PEG10, PLAGL1, KCNQ1OT1, PEG3, GRB10 и PEG1/MEST) в расширенных выборках эмбрионов подтвердило результаты микрочипового анализа как в отношении частот эпимутаций, так и в отношении преобладания соматического гипометилирования аллелей материнского происхождения. Установлено, что потери беременности сопровождаются мультилокусными дефектами метилирования импринтированных генов, частота которых также статистически значимо повышена в плацентарных тканях спонтанных абортусов от женщин с привычным невынашиванием.

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Bocharova A.V., Stepanov V.A., Marusin A.V., Kharkov V.N., Vagaitseva K.V., Fedorenko O.Yu., Bokhan N.A., Semke A.V., Ivanova S.A.
Russian Journal of Genetics. 2017. 53(1), 139–146.
DOI: 10.1134/S1022795417010033

A replicative analysis of associations of 15 SNPs located in the regions of 11 genes (TCF4, VRK2, NOTCH4, ZNF804A, AGBL1, RELN, ZFP64P1, KCNB2, CSMD1, CPVL, NRIP1) and three intergenic regions (SLCO6A1/LINCOO491, LOC105376248/LOC105376249, SPA17/NRGN) with schizophrenia was conducted in the Russian population of the Siberian region. These SNPs were previously identified in genome-wide association studies (GWAS) of schizophrenia and cognitive abnormalities. The present study confirmed associations of KCNB2 rs2247572, CSMD1 rs2616984, and intergenic rs12807809 located in SPA17/NRGN with schizophrenia. It was established that the frequency of the CSMD1 rs2616984 G/G genotype was higher in patients compared to the control group (OR = 1.73; CI: 1.14–2.62; р = 0.0337). The frequencies of the KCNB2 rs2247572 TT genotype (OR = 0.41; CI: 0.20–0.87; р = 0.0485) and intergenic rs12807809 CT genotype located in SPA17/NRGN (OR = 0.70; CI: 0.53–0.94; р = 0.0464) were significantly decreased in patients compared to the control group.

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А.В. Бочарова, В.А. Степанов, А.В. Марусин, В.Н. Харьков, К.В. Вагайцева, О.Ю. Федоренко, Н.А. Бохан, А.В. Семке, С.А. Иванова.
Генетика. 2017. Т. 53. № 1. С. 100-108
DOI: 10.7868/S0016675817010039

Проведено исследование ассоциаций полиморфных локусов, расположенных в генах (TCF4, VRK2, NOTCH4, ZNF804A, AGBL1, RELN, ZFP64P1, KCNB2, CSMD1, CPVL, NRIP1) и межгенных участках (SLCO6A1/LINCOO491, LOC105376248/LOC105376249, SPA17/NRGN) с предрасположенностью к шизофрении в русской популяции Сибирского региона. По данным полногеномных ассоциативных исследований (GWAS) эти маркеры показали ассоциации с риском развития шизофрении и нарушениями когнитивных способностей. В результате нашей работы были подтверждены ассоциации полиморфных вариантов rs2247572 гена KCNB2 и rs2616984 гена CSMD1, а также rs12807809 межгенного участка SPA17/NRGN с риском развития шизофрении. Выявлено, что генотип GG по полиморфному варианту rs2616984 гена CSMD1 статистически значимо чаще встречается у больных, чем в контроле (OR = 1.73; CI: 1.14-2.62; р = 0.0337). Установлено, что частота генотипа ТТ полиморфного маркера rs2247572 гена KCNB2 была статистически значимо ниже у больных, чем в группе контроля (OR = 0.41; CI: 0.20-0.87; р = 0.0485), то же самое было отмечено для частоты генотипа CT полиморфного локуса rs12807809 межгенного участка SPA17/NRGN (OR = 0.70; CI: 0.53- 0.94; р = 0.0464).

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Fedorenko O.Y., Loonen A.J.M., Vyalova N.M., Boiko A.S., Pozhidaev I.V., Osmanova D.Z., Rakhmazova L.D., Bokhan N.A., Ivanov M.V., Freidin M.B. и др.
Physiology and Pharmacology. 2017. 21(1), 25-33
DOI: 10.1111/bcpt.13323

Aripiprazole treatment in schizophrenic patients was previously associated with lower or normalized prolactin levels. Genetic variants in cytochrome P450 (CYP) (CYP2D6), dopamine receptor (DRD2, DRD3) and serotonin receptor (HTR2A, HTR2C) genes were previously associated with antipsychotic-induced hyperprolactinaemia. Our aim was to evaluate whether aripiprazole affects prolactin secretion and its relationship with pharmacogenetics. Thirty-one healthy volunteers receiving a 10-mg single oral dose of aripiprazole were genotyped for 12 polymorphisms in CYP2D6, DRD2, DRD3, HTR2A and HTR2C genes by qPCR. Aripiprazole and dehydro-aripiprazole plasma concentrations were measured by HPLC-MS/MS. Prolactin concentrations of the 31 volunteers taking aripiprazole and 12 volunteers receiving ibuprofen were determined by ELISA. Prolactin concentrations after ibuprofen intake were considered as control, since it is known to cause no effect. Prolactin concentrations were slightly higher in the aripiprazole group compared to the ibuprofen group. All prolactin pharmacokinetic parameters were higher in females than in males. CYP2D6 poor and intermediate metabolizers had notably higher prolactin Cmax and AUC0-12 than normal and ultrarapid metabolizers. The DRD3 rs6280 polymorphism affected prolactin levels: volunteers carrying Ser/Ser genotype had significantly lower prolactin levels than volunteers carrying the Gly allele. Furthermore, HTR2C rs3813929 C/C homozygotes had significantly lower prolactin levels than T allele carriers. Nevertheless, aripiprazole did increase prolactin levels compared to ibuprofen.

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Triska P., Chekanov, N., Stepanov V., Khusnutdinova E.K., Kumar G.P.A., Akhmetova V., Babalyan K., Boulygina E., Kharkov V., Gubina M., Khidiyatova I., Khitrinskaya I., Khrameeva E.E., Khusainova R., Konovalova N., Litvinov S., Marusin A., Mazur A.M., Puzyrev V., Ivanoshchuk D., Spiridonova M., Teslyuk A., Tsygankova S., Triska M., Trofimova N., Vajda E., Balanovsky O., Baranova A., Skryabin K., Tatarinova T.V., Prokhortchouk E.
BMC Genetics. 2017. 18(Suppl 1), 110.
DOI: 10.1186/s12863-017-0578-3

Background: The history of human populations occupying the plains and mountain ridges separating Europe from Asia has been eventful, as these natural obstacles were crossed westward by multiple waves of Turkic and Uralic-speaking migrants as well as eastward by Europeans. Unfortunately, the material records of history of this region are not dense enough to reconstruct details of population history. These considerations stimulate growing interest to obtain a genetic picture of the demographic history of migrations and admixture in Northern Eurasia.

Results: We genotyped and analyzed 1076 individuals from 30 populations with geographical coverage spanning from Baltic Sea to Baikal Lake. Our dense sampling allowed us to describe in detail the population structure, provide insight into genomic history of numerous European and Asian populations, and significantly increase quantity of genetic data available for modern populations in region of North Eurasia. Our study doubles the amount of genome-wide profiles available for this region. We detected unusually high amount of shared identical-by-descent (IBD) genomic segments between several Siberian populations, such as Khanty and Ket, providing evidence of genetic relatedness across vast geographic distances and between speakers of different language families. Additionally, we observed excessive IBD sharing between Khanty and Bashkir, a group of Turkic speakers from Southern Urals region. While adding some weight to the ""Finno-Ugric"" origin of Bashkir, our studies highlighted that the Bashkir genepool lacks the main ""core"", being a multi-layered amalgamation of Turkic, Ugric, Finnish and Indo-European contributions, which points at intricacy of genetic interface between Turkic and Uralic populations. Comparison of the genetic structure of Siberian ethnicities and the geography of the region they inhabit point at existence of the ""Great Siberian Vortex"" directing genetic exchanges in populations across the Siberian part of Asia. Slavic speakers of Eastern Europe are, in general, very similar in their genetic composition. Ukrainians, Belarusians and Russians have almost identical proportions of Caucasus and Northern European components and have virtually no Asian influence. We capitalized on wide geographic span of our sampling to address intriguing question about the place of origin of Russian Starovers, an enigmatic Eastern Orthodox Old Believers religious group relocated to Siberia in seventeenth century. A comparative reAdmix analysis, complemented by IBD sharing, placed their roots in the region of the Northern European Plain, occupied by North Russians and Finno-Ugric Komi and Karelian people. Russians from Novosibirsk and Russian Starover exhibit ancestral proportions close to that of European Eastern Slavs, however, they also include between five to 10 % of Central Siberian ancestry, not present at this level in their European counterparts.

Conclusions: Our project has patched the hole in the genetic map of Eurasia: we demonstrated complexity of genetic structure of Northern Eurasians, existence of East-West and North-South genetic gradients, and assessed different inputs of ancient populations into modern populations.

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Stepanov V., Marusin A., Vagaitseva K., Bocharova A., Makeeva O.
Genetics Research International. 2017, 6293826
DOI: 10.1155/2017/6293826

Recently, genetic markers rs10503253 and rs2616984 in the CUB and Sushi multiple domains-1 (CSMD1) gene have been reported to be associated with schizophrenia and cognitive functions in genome-wide association studies. We examined the associations of the above SNPs with cognitive performance evaluated by the Montreal Cognitive Assessment (MoCA) tool in a cohort of the normal elderly from the Russian population. Significant association of rs2616984 genotypes with the MoCA scores was found using nonparametric analysis. No association of rs10503253 with MoCA scores was observed using both parametric and nonparametric statistics. Significant combined effect of two-locus CSMD1 genotypes on MoCA scores was demonstrated by median test. Allele ""A"" and genotype ""AA"" of rs2616984 were significantly associated with the lower MoCA scores in comparison of 1st and 4th quartiles of MoCA total score distribution. The results suggest that genetic variants in CSMD1 gene are likely a part of genetic component of cognitive performance in the elderly.

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Korablev A.N., Serova I.A., Serov O.L.
BMC Genetics. 2017. 18(Suppl 1), 112
DOI: 10.1186/s12863-017-0582-7

Background: Copy Number Variation (CNV) of the human CNTN6 gene (encoding the contactin-6 protein), caused by deletions or duplications, is responsible for severe neurodevelopmental impairments, often in combination with facial dysmorphias. Conversely, deleterious point mutations of this gene do not show any clinical phenotypes. The aim of this study is to generate mice carrying large deletions, duplications and inversions involving the Cntn6 gene as a new experimental model to study CNV of the human CNTN6 locus.

Results: To generate large chromosomal rearrangements on mouse chromosome 6, we applied CRISPR/Cas9 technology in zygotes. Two guide RNAs (gRNAs) (flanking a DNA fragment of 1137 Mb) together with Cas9 mRNA and single-stranded DNA oligonucleotides (ssODN) were microinjected into the cytoplasm of 599 zygotes of F1 (C57BL x CBA) mice, and 256 of them were transplanted into oviducts of CD-1 females. As a result, we observed the birth of 41 viable F0 offspring. Genotyping of these mice was performed by PCR analysis and sequencing of PCR products. Among the 41 F0 offspring, we identified seven mice with deletions, two animals carrying duplications of the gene and four carrying inversions. Interestingly, two F0 offspring had both deletions and duplications. It is important to note that while three of seven deletion carriers showed expected sequences at the new joint sites, in another three, we identified an absence of 1-10 nucleotides at the CRISPR/Cas9 cut sites, and in one animal, 103 bp were missing, presumably due to error-prone non-homologous end joining. In addition, we detected the absence of 5 and 13 nucleotides at these sites in two F0 duplication carriers. Similar sequence changes at CRISPR/Cas9 cut sites were observed at the right and left boundaries of inversions. Thus, megabase-scale deletions, duplications and inversions were identified in 11 F0 offspring among 41 analyzed, i.e., approximately 25% efficiency. All genetically modified F0 offspring were viable and able to transmit these large chromosomal rearrangements to the next generation.

Conclusions: Using CRISPR/Cas9 technology, we created mice carrying megabase-scale deletions, duplications, and inversions involving the full-sized Cntn6 gene. These mice became founders of new mouse lines, which may be more appropriate experimental models of CNV in the human 3p26.3 region than Сntn6 knockout mice.

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Polonikov A., Kharchenko A., Bykanova M., Sirotina S., Ponomarenko I., Bocharova A., Vagaytseva K., Stepanov V., Bushueva O., Churnosov M., Solodilova M.
Gene. 2017. 627, 451-459.
DOI: 10.1016/j.gene.2017.07.004

Epoxyeicosatrienoic acids (EETs) are important vasoactive products of arachidonic acid metabolism with a wide range of biological actions in the cardiovascular system. The present study investigated whether single nucleotide polymorphisms (SNP) of genes coding cytochrome P450 2C subfamily, enzymes involved in biosynthesis of EETs, are associated with the risk of coronary heart disease (CHD). A total of 1255 unrelated Russian subjects comprising 561 patients with angiographically diagnosed CHD and 694 age- and sex-matched healthy subjects were included in the study. DNA samples from all study participants were genotyped for six common SNPs rs7909236, rs1934953 of CYP2C8, rs9332242, rs4918758 and rs61886769 of CYP2C9 and rs4244285 of CYP2C19 using by the Mass-ARRAY 4 system. SNP rs4918758 of CYP2C9 was associated with decreased risk of CHD (codominant model) at a borderline significance with odds ratio adjusted for sex and age 0.61 (95% CI: 0.41-0.92, P=0.038, Q=0.20). SNP rs9332242 of CYP2C9 showed a trend towards association with increased CHD risk in cigarette smokers (P=0.049, Q=0.29). Log-likelihood ratio test (LRT) pointed out epistatic interactions between rs9332242 and rs61886769 of CYP2C9 (codominant model, Pinteraction=0.02), however, this P-value did not survive after correction for multiple tests. Bioinformatic analysis revealed a regulatory potential for a majority of the investigated SNPs. Our preliminary results demonstrate that polymorphisms of genes encoding CYP2C subfamily represent potential genetic markers of CHD susceptibility. Further studies are required to substantiate the contribution of these genes to the disease risk.

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Sahakyan H., Kashani B.H., Tamang R., Kushniarevich A., Francis A., Costa M.D., Pathak A.K., Khachatryan Z., Sharma I., van Oven M., Parik J., Hovhannisyan H., Metspalu E., Pennarun E., Karmin M., Tamm E., Tambets K., Bahmanimehr A., Reisberg T., Reidla M., Achilli A., Olivieri A., Gandini F., Perego U.A., Al-Zahery N., Houshmand M., Sanati M.H., Soares P., Rai E., Šarac J., Šarić T., Sharma V., Pereira L., Fernandes V., Černý V., Farjadian S., Singh D.P., Azakli H., Üstek D., Ekomasova Trofimova N., Kutuev I., Litvinov S., Bermisheva M., Khusnutdinova E.K., Rai N., Singh M., Singh V.K., Reddy A.G., Tolk H.V., Cvjetan S., Lauc L.B., Rudan P., Michalodimitrakis E.N., Anagnou N.P., Pappa K.I., Golubenko M.V., Orekhov V., Borinskaya S.A., Kaldma K., Schauer M.A., Simionescu M., Gusar V., Grechanina E., Govindaraj P., Voevoda M., Damba L., Sharma S., Singh L., Semino O., Behar D.M., Yepiskoposyan L., Richards M.B., Metspalu M., Kivisild T., Thangaraj K., Endicott P., Chaubey G., Torroni A., Villems R.
Scientific Reports. 2017. 7, 46044.
DOI: 10.1038/srep46044

Human mitochondrial DNA haplogroup U is among the initial maternal founders in Southwest Asia and Europe and one that best indicates matrilineal genetic continuity between late Pleistocene hunter-gatherer groups and present-day populations of Europe. While most haplogroup U subclades are older than 30 thousand years, the comparatively recent coalescence time of the extant variation of haplogroup U7 (~16-19 thousand years ago) suggests that its current distribution is the consequence of more recent dispersal events, despite its wide geographical range across Europe, the Near East and South Asia. Here we report 267 new U7 mitogenomes that - analysed alongside 100 published ones - enable us to discern at least two distinct temporal phases of dispersal, both of which most likely emanated from the Near East. The earlier one began prior to the Holocene (~11.5 thousand years ago) towards South Asia, while the later dispersal took place more recently towards Mediterranean Europe during the Neolithic (~8 thousand years ago). These findings imply that the carriers of haplogroup U7 spread to South Asia and Europe before the suggested Bronze Age expansion of Indo-European languages from the Pontic-Caspian Steppe region.

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Polonikov A, Bykanova M, Ponomarenko I, Sirotina S, Bocharova A, Vagaytseva K, Stepanov V, Churnosov M, Bushueva O, Solodilova M, Shvetsov Y, Ivanov V.
Clinical and Experimental Hypertension. 2017. 39(4), 306-311
DOI: 10.1080/10641963.2016.1246562

Numerous studies demonstrated an importance of cytochrome P-450 epoxygenase pathway of arachidonic acids metabolism for the pathogenesis of essential hypertension (EH). The present study was designed to investigate whether common single-nucleotide polymorphisms (SNP) of CYP2C gene subfamily such as CYP2C8 (rs7909236 and rs1934953), CYP2C9 (rs9332242), and CYP2C19 (rs4244285) are associated with susceptibility to EH in Russian population. A total of 816 unrelated Russian individuals comprising 425 EH patients and 391 normotensive controls were included into the study. Genotyping of SNPs was performed using the MassARRAY 4 system. SNP rs7909236 of CYP2C8 was significantly associated with increased risk of EH (OR adjusted for sex and age was 2.99 95% CI 1.39-6.44, P = 0.005). SNPs rs1934953 CYP2C8 and rs4244285 of CYP2C19 showed association with EH risk but at a borderline statistical significance (P ≤ 0.04). Combination of genotypes CYP2C8 rs7909236 TT and CYP2C19 rs4244285 GG was associated with increased EH risk (OR 3.34 95%CI 1.48-7.51, P = 0.004). Genotype-phenotype correlation analysis showed that the levels of CYP2C8 mRNA were significantly correlated with SNP rs7909236 (P = 0.01). in silico functional prediction analysis revealed the functionality of majority of investigated SNPs. Thus, genes of CYP2C subfamily are important genetic determinants of susceptibility to essential hypertension in Russians.

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Larruga J.M., Marrero P., Abu-Amero K.K., Golubenko M.V., Cabrera V.M.
BMC Evolutionary Biology. 2017. 17(1), 115.
DOI: 10.1186/s12862-017-0964-5

Background: The colonization of Eurasia and Australasia by African modern humans has been explained, nearly unanimously, as the result of a quick southern coastal dispersal route through the Arabian Peninsula, the Indian subcontinent, and the Indochinese Peninsula, to reach Australia around 50 kya. The phylogeny and phylogeography of the major mitochondrial DNA Eurasian haplogroups M and N have played the main role in giving molecular genetics support to that scenario. However, using the same molecular tools, a northern route across central Asia has been invoked as an alternative that is more conciliatory with the fossil record of East Asia. Here, we assess as the Eurasian macrohaplogroup R fits in the northern path.

Results: Haplogroup U, with a founder age around 50 kya, is one of the oldest clades of macrohaplogroup R in western Asia. The main branches of U expanded in successive waves across West, Central and South Asia before the Last Glacial Maximum. All these dispersions had rather overlapping ranges. Some of them, as those of U6 and U3, reached North Africa. At the other end of Asia, in Wallacea, another branch of macrohaplogroup R, haplogroup P, also independently expanded in the area around 52 kya, in this case as isolated bursts geographically well structured, with autochthonous branches in Australia, New Guinea, and the Philippines.

Conclusions: Coeval independently dispersals around 50 kya of the West Asia haplogroup U and the Wallacea haplogroup P, points to a halfway core area in southeast Asia as the most probable centre of expansion of macrohaplogroup R, what fits in the phylogeographic pattern of its ancestor, macrohaplogroup N, for which a northern route and a southeast Asian origin has been already proposed.

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